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Table 5 Summarizes the Plackett–Burman experimental design utilized for screening the culture conditions affecting PHB production

From: Optimization and characterization of polyhydroxybutyrate produced by Halomonas meridiana using orange peel waste

Run

A

B

C

D

E

F

G

H

J

K

L

PHB (g/L)

1

−1

1

−1

1

1

−1

1

1

1

−1

−1

1.85

2

−1

1

1

−1

1

1

1

−1

−1

−1

1

2.1

3

1

−1

−1

−1

1

−1

1

1

−1

1

1

2.2

4

1

1

1

−1

−1

−1

1

−1

1

1

−1

1.47

5

−1

−1

1

−1

1

1

−1

1

1

1

−1

2.1

6

−1

−1

−1

1

−1

1

1

−1

1

1

1

0.78

7

0

0

0

0

0

0

0

0

0

0

0

1.92

8

1

−1

1

1

1

−1

−1

−1

1

−1

1

1.61

9

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

1.47

10

0

0

0

0

0

0

0

0

0

0

0

1.92

11

1

1

−1

1

1

1

−1

−1

−1

1

−1

0.76

12

0

0

0

0

0

0

0

0

0

0

0

1.96

13

−1

1

1

1

−1

−1

−1

1

−1

1

1

0.55

14

1

−1

1

1

−1

1

1

1

−1

−1

−1

0.43

15

1

1

−1

−1

−1

1

−1

1

1

−1

1

1.86

  1. A, Orange peel concentration (g/L); B, Temperature (oC); C, Sodium chloride (%); D, Incubation time (hr.); E, Inoculum size (%) (v/v); F, Agitation rate (rpm); G, MgCl2.6H2O (g/L); H, MgSO4.7H2O (g/L); J, (NH4)2HPO4 (g/L); K, Methanol (co-substrate) (g/L); L, Initial pH