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Table 2 The levels of each factor in the plackett–burman experimental design

From: Optimization and characterization of polyhydroxybutyrate produced by Halomonas meridiana using orange peel waste

Factor code

Factor

Level value

Low (− 1)

High (+ 1)

A

Orange peel concentration (g/L)

600

1000

B

Temperature (oC)

35

40

C

Sodium chloride (%)

10

15

D

Incubation time (hr.)

72

96

E

Inoculum size (%) (v/v)

2

10

F

Agitation rate (rpm)

120

180

G

MgCl2.6H2O (g/L)

1.35

4.05

H

MgSO4.7H2O (g/L)

1.7

5.1

J

(NH4)2HPO4 (g/L)

1.0

3.0

K

Methanol (co-substrate) (g/L)

2.0

4.0

L

Initial pH

5

8