Table 1 Primers for qPCR amplification used in this study
Gene | Primer set | Sequence (5′–3′) |
|---|---|---|
IFN-É‘ | Forward | CTGGCCAACCTGCTCTCTAG |
| Â | Reverse | CTCCTGCGGGAATCCAAAGT |
IFN-β | Forward | CAGCAATTTTCAGTGTCAGAAGC |
| Â | Reverse | TCATCCTGTCCTTGAGGCAGT |
ISG15 | Forward | GAGCTAGAGCCTGCAGCAAT |
| Â | Reverse | TCACGGACACCAGGAAATCG |
CXCL10 | Forward | TGCCCTGACAAACTAATGAGC |
| Â | Reverse | CAAGGCATATTCTGCACCAG |
IFIT2 | Forward | ATGCCACTTCACCTGGAACC- |
| Â | Reverse | CTTCGGCTTCCCCTAAGCAT |
LC3B | Forward | TGTCCGACTTATTCGAGAGCAGCA |
| Â | Reverse | TGTGTCCGTTCACCAACAGGAAGA |
ATG5 | Forward | GTGACTGGACTTACGGTGGG |
| Â | Reverse | GCCAAACTTCTTGCTCCCGA |
ATG8 | Forward | TCTGAGTCAAGAGGAGGGGT |
| Â | Reverse | CCGGGGCCGAAATAGCTTAG |
ATG12 | Forward | GCGAGCGGGTTCCCATT |
| Â | Reverse | CATTCCCAGCCATCGCCTAT |
Beclin-1 | Forward | TCAAGGCGTCACTTCTGGG |
| Â | Reverse | TTCTTTAGGCCCCGACGCTC |
GAPDH | Forward | GGTGTGAACCATGAGAAGTATGA |
| Â | Reverse | GAGTCCTTCCACGATACCAAG |
PEDV-N | Forward | GCAAAGACTGAACCCACTAAT |
| Â | Reverse | GCCTCTGTTGTTACTTGGAG |