Table 2 Gene names and product sizes for qRT-PCR or PCR
Gene name | Primer sequence(5’-3’) | Product size (bp) | Methods |
|---|---|---|---|
cyp | F: TATTGCTTGCCGCCACAGAACC R: GCGTCTCTGCGATTGCCTGAG | 132 | qRT-PCR |
16SrRNA | F: GTAGTCCACGCTGTAAACGA R: GAATTAAACCACATGCTCCA | 173 | qRT-PCR |
cyp | F: CCGGAATTCCGGAGCGGACGAAAGGGTGGAAT R: GGCGAGCTCGCCCTGGCGAAGATCAGTGAGGC | 1221 | PCR |
GAPDH | F: ACCCAGAAGACTGTGGATGG R: ACACATTGGGGGTAGGAACA | 146 | qRT-PCR |
IL-6 | F: TTTCCTCTGGTCTTCTGGAG R: CTGAAGGACTCTGGCTTTGT | 92 | qRT-PCR |
occludin | F: TTGAAAGTCCACCTCCTTACAGA R: CCGGATAAAAAGAGTACGCTGG | 184 | qRT-PCR |