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Fig. 3 | BMC Microbiology

Fig. 3

From: Prophylactic administration of overproducing-abscisic acid Bacillus licheniformis attenuated DSS-induced colitis in mice by regulating the gut microbiota and immune activity

Fig. 3

Prophylactic administration of the Bl-cyp strain more effectively decreased DSS-induced epithelial barrier damage than other treatments. a Representative pictures of the goblet cells and epithelial cells in colonic epithelial layer from the control, Bl, Bl-pET28a, Bl-cyp, DSS and Nfb groups after immunofluorescence staining (scale bars,100 μm; magnification, 100x or 400x). The epithelial cell markers-EpCAM and CD326 appeared green and the goblet cell marker, MUC2 was red, and the cell nuclei stained by DAPI was blue. b Comparison of the proportion of goblet cells to epithelial cells in the DSS group with those in the control, Nfb, Bl, Bl-cyp and Bl-pET28a groups, and comparison of the proportion in the Bl-cyp group with those in the Bl-pET28a and Bl groups. c Comparison of the serum LPS levels in the DSS group with those in the control, Nfb, Bl, Bl-cyp and Bl-pET28a groups, and comparison of those in the Bl-cyp group with those in the Bl-pET28a and Bl groups. d Comparison of the mRNA expression of the occludin gene in the colon tissues of the DSS group with those in the control, Nfb, Bl, Bl-cyp and Bl-pET28a groups, and comparison of those in the Bl-cyp group with those in the Bl-pET28a and Bl group. Data represent the means ± SD, n = 6–8 per group. * P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant. Statistical significance was assessed using a one-way ANOVA, followed by Tukey’s test

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BMC Microbiology

ISSN: 1471-2180

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