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Fig. 4 | BMC Microbiology

Fig. 4

From: The natural symbiotic bacterium Enterococcus faecalis LX10 drives Bombyx mori refractoriness to Nosema bombycis infection via the secretion of enterococcin

Fig. 4

Subcellular localization of the BmN transgenic cell line and the expression level of EntLX determined via qPCR. (a) Subcellular localization of EntLX as demonstrated by immunofluorescence via confocal microscopy.cell nucleus (DAPI; blue), anti-EntLX antibody (EntLX; red), and merged images. (b) BmN cells were infected with N. bombycis at 107 spores/mL, and the cell infection rate was determined via light microscopy. The percentage of N. bombycis infection was calculated for the CK group and transfected cell groups. (c) DNA was extracted from the control and transfected cell groups, and N. bombycis intracellular burden was estimated via qPCR. The infection rate and number of spore copies of N. bombycis were significantly reduced. Four asterisks (****) represent significant differences (P < 0.0001). (d, e) RT‒PCR analysis of the specific expression of EntLX by Ent F/R in BmN transgenic cells. All assays were carried out in triplicate

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BMC Microbiology

ISSN: 1471-2180

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