Fig. 2

Alkylation of EntLX and the cleavage process involving GelE and anti-N. bombycis activity. (a) Reduction and alkylation of the supernatant by 2-mercaptoethanol and 4-vinylpyridine reduce the activity against N. bombycis. (b) GelE is necessary for the inhibition of N. bombycis germination. EntLX136 (50 µg/ml) was incubated with GelE (1.25, 2.5, 5, 10, 15 and 20 µg/ml). (c) Cleavage process of EntLX by GelE based on SDS‒PAGE