Fig. 6

ALE with C1* ΔTRP ΔtrpP via repetitive batch cultures. The fully automated experiment was performed on a Mini Pilot Plant and each batch was started with freshly stored CGXII medium (111 mM glucose, 0.5 g L− 1l-tryptophan). After the predefined backscatter of BS = 25 was reached, the next batch was inoculated with cells from the previous batch to an OD600 of 0.5. (A) Online backscatter measurements from a representative ALE from three independent replicates. (B) Evolution of the maximum specific growth rates along the repetitive batches