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Fig. 4 | BMC Microbiology

Fig. 4

From: ALE reveals a surprising link between [Fe-S] cluster formation, tryptophan biosynthesis and the potential regulatory protein TrpP in Corynebacterium glutamicum

Fig. 4

Characterization of the influence of trpP on amino acid formation in C. glutamicum. Cultivation of WT TRP+++-derived trpP deletion (A) and plasmid-based overexpression (B) strains regarding their growth, l-tryptophan accumulation and l-valine accumulation. The strains were cultivated in biological triplicates (quadruplicates for the plasmid-harboring strains) in 50 ml CGXII media supplemented with 111 mM glucose in 500 ml baffled shake flasks. The cultivation started with an OD600 = 0.8, and 200 µM IPTG and 25 µg/ml kanamycin were added to the plasmid-harboring strains. The mean values are shown as diamonds, and the standard deviations are shown as lines

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BMC Microbiology

ISSN: 1471-2180

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