Fig. 1

Growth of the trp operon and trpP deletion and complemented strains. (A) Growth of the WT and two l-tryptophan auxotrophic strains. (B) Growth of WT and WT ΔtrpP. (C) Plasmid-based complementation of WT ΔtrpP with pPREx2-trpP. All cultures were grown in defined CGXII media supplemented with 111 mM d-glucose and 0.5 mM l-tryptophan when necessary. Kanamycin (25 µg mL^− 1) was added for strains harboring a plasmid. The backscatter data were normalized based on the maximum value recorded for each WT in the corresponding experiment. The mean values of biological triplicates are shown as lines and standard deviations are shown as shaded areas