Fig. 3

Analysis of OprD mutations in amino acids. (a) Analysis of mutant amino acids based on OprD structure. The figure indicates the extracellular, intracellular, and outer membrane regions. The amino acids highlighted in orange represent the mutated amino acids in the summarized CROPA’s OprD. (b) Alignment of amino acid variation based on OprD sequence. Scale numbers above of the alignment refer to amino acid positions in variant OprD β-Strands (according to PDB structure 3SY7) are underlined. CLUSTALW (https://www.genome.jp/tools-bin/clustalw) and ESPript 3.0 (http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi) were used to compare OprD sequence of PAO1 with CROPA-02, CROPA-03, CROPA-04, CROPA-06, CROPA-08, CROPA-09. Residues different from PAO1 OprD are marked with red pentagons