Virulence genes and antibiotic resistance profiling of staphylococcus species isolated from mastitic dairy cows in and around Bahir dar, Ethiopia

Table 2 Amount of PCR Component in µl and annealing temperature in ℃

Amount of the PCR components in µl
	NFW	10x Reaction Buffer			Primer (FW+RV)	Taq. DNA Polymerase	Sample DNA
Virulence genes			MgCl₂	dNTPS				Annealing T.M in ℃ and No. of Cycles
icaD	12.1	2	1.5	0.2	1	0.2	3	48/35 cycles
Coa	11.6	2	2	0.2	1	0.2	3	55/35 cycles
seA	11.6	2	2	0.2	1	0.2	3	53/35 cycles
seB	11.6	2	2	0.2	1	0.2	3	5335 cycles /
seC	11.6	2	2	0.2	1	0.2	3	55/35 cycles
Hlb	12.1	2	1.5	0.2	1	0.2	3	55/35 cycles
Hla	11.6	2	2	0.2	1	0.2	3	59/35 cycles
PVL	12.1	2	1.5	0.2	1	0.2	3	55/35 cycles
mecA	12.1	2	1.5	0.2	1	0.2	3	51/35 cycles
mecC	12.1	2	1.5	0.2	1	0.2	3	51/35 cycles
tsst-1	11.6	2	2	0.2	1	0.2	3	55/35 cycles

*NFW nuclease-free water, dNTPS deoxyneoclotide tri phosphate, FW forward primer, RV reverse primer, DNA deoxyneoclotide tri phosphate, TM temperature, icaD intracellular adhesion, Coa coagulase gene, sea enterotoxin A gene, seb enterotoxin B, sec entrotoxine c, Hlb hemolaysin B, Hla hemolaysin A, PVL gene encoding pento valentine leukocidine, mecA and mecC gene encoding methicillin resistance, TSSt-1 toxic shock syndrome toxine-1

ISSN: 1471-2180