Virulence genes and antibiotic resistance profiling of staphylococcus species isolated from mastitic dairy cows in and around Bahir dar, Ethiopia

Table 1 The sequences of primers used for molecular identification

Virulence factor	Gene		Primer Sequence (5'- 3')	Primer length	Amplified Size (Bp)	Reference
Intercellular adhesion	icaD	Forward	AAACGTAAGAGAGGTGG	17	381	[28]
factor D	icaD	Reverse	GGCAATATGATCAAGATAC	19
Coagulase	coa	Forward	ATGGAGATGCTGGTACAGG	19	514	[29]
	coa	Reverse	GCTTCCGATTGTTCGATGC	19
	sea	Forward	ATTAACCGAAGGTTCTGTAGA	21	552	[30]
	sea	Reverse	TTGCGTAAATCTGAATT	17
Enterotoxins	seb	Forward	TGTATGTATGGAGGTGTAAC	20	270	[30]
	seb	Reverse	ATAGTGACGAGTTAGGTA	18
	seC	Forward	CTTGTATGTATGGAGGAATAACAA	24	284	[31]
	seC	Reverse	CTTGTATGTATGGAGGAATAACAA	21
Antibiotic resistance	mecA	Forward	TCCAGATTACAACTTCACCAGG	22	162	[30]
Gene	mecA	Reverse	CCACTTCATATCTTGTAACG	20
	mecC	Forward	GAAAAAAAGGCTTAGAACGCCTC	23	138	[30]
	mecC	Reverse	GAAGATCTTTTCCGTTTTCAGC	20
Hemolysins	Hlb	Forward	GCCAAAGCCGAATCTAAGAAAG	22	495	[32]
	Hlb	Reverse	ATCATGTCCAGCACCACAA	19
	Hla	Forward	GGTTTAGCCTGGCCTTC	17	550	[33]
	Hla	Reverse	CATCACGAACTCGTTCG	17
Toxic shock syndrome		Forward	ACCCCTGTTCCCTTATCATC	20	326	[34]
toxin-1	tsst-1	Reverse	TTTTCAGTATTTGTAACGCC	20
Cytotoxic genes	Pvl	Forward	TTACACAGTTAAATATGAAGTGAACTGGA	29	118	[35]
Cytotoxic genes	Pvl	Reverse	AGCAAAAGCAATGCAATTGATG	22

ISSN: 1471-2180