Fig. 5

Heatmap analysis of co-occurrence between Acanthamoeba and K. pneumoniae. The heatmap presents the co-occurrence patterns of Acanthamoeba and K. pneumoniae across the sampling sites. It highlights locations where both microorganisms were detected, as well as areas where only one was present. The heatmap allows for the identification of potential environmental or ecological factors that may influence the distribution of these organisms and their co-existence in freshwater systems