Fig. 4

The regulatory effect of FMT on hepatic lipid metabolism. a-b, Score plots (a) and 2D score plots (b) of PCA for discriminating the hepatic metabolome in mice from the AllC, AutC and cycHFD groups; n = 6 per group. c-d, An OPLS-DA score plot (c) and one hundred permutations (d) were generated and plotted for the AutC and cycHFD groups; n = 6 per group. e-f, An OPLS-DA score plot (e) and one hundred permutations (f) were generated and plotted for the AllC and cycHFD groups; n = 6 per group. g-h, Differential lipid metabolites between the AutC and cycHFD groups are shown in a volcano plot (g), and the respective composition ratios are shown in a pie chart (h). n = 6 per group. i-j, Differential lipid metabolites between the AllC and cycHFD groups (i) and the respective composition ratio (j). n = 6 per group. k-l, Top 20 VIP scores between the AutC and cycHFD groups (k) and between the AllC and cycHFD groups (l). m, Metabolic pathway enrichment analyses of differentially abundant metabolites. n-o, Western blots for FASN in liver tissue (n), and relative quantification of FASN at week 15; n = 3 per group. β-Actin served as the loading control. Full-length blots are presented in Supplementary Fig. 1. The data are presented as the mean ± s.e.m. *P < 0.05, **P < 0.01 vs. the cycHFD group