Fig. 3

Increased energy expenditure and changes in serum factor levels. a-f, O2 consumption (a, b), energy, expenditure (c, d), food intake (e) and the RER (f) over 36 h at week 15; n = 3 per group. g-h, Western blots for UCP1 in brown adipose tissue (g) and relative quantification of UCP1 at week 15; n = 3 per group. β-Actin served as the loading control Full-length blots are presented in Supplementary Fig. 1. i-l, The levels of serum IL-6 (i), TNF-a (j), leptin (k) and adiponectin (l) at week 15; n = 6 in each group. The data are presented as the mean ± s.e.m. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. the ND group; *P < 0.05, **P < 0.01 vs. the cycHFD group