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Table 2 Efflux pump primers used in Real-Time PCR

From: Analysis of relative genes expression and mutation of pstB and efpA efflux pumps in Mycobacterium simiae isolates from suspected tuberculosis patients by using quantitative Real-time PCR

target sequence

primers

( ̍ 3 ̍5)

Bp

Ref

PstB

Forward

F: 5'- GGTAAAGGACGCAGCTACAAG -3'

bp 311

This study

 

Reverse

R: 5' ATAACTCTCCCCATTGCAGGT -3'

  

EfpA

Forward

Reverse

F: 5' AGGAACACTTGCTCTTTGAGCC -3'

R: 5' CAAATCACTCCCAAGCTGGC -3'

bp 206

This study

16SrDNA

Forward

Reverse

F: 5' CTTAACACATCGAAC -3'

R: 5' GTATCTCAGTCCCAGTGTG -3'

bp 278

7