From: Analysis of relative genes expression and mutation of pstB and efpA efflux pumps in Mycobacterium simiae isolates from suspected tuberculosis patients by using quantitative Real-time PCR
target sequence
Primers
( ̍ 3 ̍5)
Bp
Ref
PstB
Forward
F: 5'- GGTAAAGGACGCAGCTACAAG -3'
bp 311
This study
Reverse
R: 5' ATAACTCTCCCCATTGCAGGT -3'
EfpA
F: 5' AGGAACACTTGCTCTTTGAGCC -3'
R: 5' CAAATCACTCCCAAGCTGGC -3'
bp 209