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Table 3 Thermal profiling (PCR conditions) of PhaB

From: Screening and characterization of PHA producing bacteria from sewage water identifying Bacillus paranthracis RSKS-3 for bioplastic production

Steps

Temperature

Time

Cycles

Initial denaturation

94 °C

1 min

X1

Final denaturation

98 °C

10 s

X32

Annealing

58 °C

15 s

Initial extension

68 °C

30 s

Final extension

68 °C

3 min

X1

Hold

12 °C

∞

X1

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BMC Microbiology

ISSN: 1471-2180

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