Table 1 Primer design for the identification of pha genes in Bacillus paranthracis RSKS-3
Primer type | Primer sequence | PHA genes | PHA specific restriction enzyme |
|---|---|---|---|
Forward | CGCCATATGATGATTGATCAAAAATTC | phaR | NdeI |
Reverse | CCGGAATTCTCATTTTTTATTTTCTGGCTTATTC | EcoRI | |
Forward | CGCCATATGATGGTTCAATTAAATGGAAAAG | phaB | NdeI |
Reverse | CCGGAATTCTTACATATATAATCCGCCGTTAATG | EcoRI | |
Forward | CGCGGATCCATGACTACATTCGTAACAGAATG | phaC | BamHI |
Reverse | CCGCTCGAGTTACTTCGAACGCTCGTCAAG | XhoI |