Improved protocols for isolation of Mycobacterium ulcerans from clinical samples

BMC Microbiology

Table 5 Comparison of mu culture recovery, using the Tika-MGIT, Petroff-MGIT and Petroff-LJ methods with mu 16S rRNA results

Decontamination and Culture Method	Number of samples with Mu 16S rRNA result		Sensitivity % (95% CI)
Decontamination and Culture Method	Positive	Negative	Sensitivity % (95% CI)
Tika-MGIT
Positive	9	1	82 (52–97)
Negative	2	9	82 (52–97)
Petroff-MGIT
Positive	6	0	55 (28–79)
Negative	5	10	55 (28–79)
Petroff-LJ
Positive	4	1	36 (15–65)
Negative	7	9	36 (15–65)
Comparison for Final culture result and Mu 16S rRNA results
Final culture results
Positive	11	2	100 (74–100)
Negative	0	8	100 (74–100)

*Mu (M. ulcerans) culture recovery was compared for Tika-MGIT, Petroff-MGIT and Petroff-LJ with a Mu 16S rRNA result (defined as a positive for any of the three methods at any of the three time points (Week 0, 2 and 4)). This was used to compute the sensitivity with a 95% CI (Confidence interval). A comparison was also made between the final Culture result and Mu 16SrRNA results

ISSN: 1471-2180