Improved protocols for isolation of Mycobacterium ulcerans from clinical samples

BMC Microbiology

Table 3 Demographic details and culture outcomes for study participants

						Microbiological Confirmation				Molecular Confirmation
Participant Code	Sex	Age	Lesion form	Lesion site	Lesion Category	Tika-MGIT	Petroff-MGIT	Petroff-LJ	Final Mu Culture Positivity	Mu 16S rRNA Positivity
BUCP1	F	13	Ulcer	LL	III	+	+	-	+	+
BUCP2	M	6	Ulcer	LL	II	+	-	+	+	+
BUCP3	F	7	Ulcer	LL	II	-	+	-	+	+
BUCP4	M	7	Ulcer	GN	III	-	-	-	-	-
BUCP5	F	27	Ulcer	AB	I	+	-	-	+	+
BUCP6	M	47	Ulcer	UL	III	-	-	-	-	-
BUCP9	M	6	Ulcer	LL	I	-	+	-	+	+
BUCP10	F	18	Ulcer	LL	II	+	-	-	+	+
BUCP12	F	63	Ulcer	BP	III	-	-	-	-	-
BUCP14	F	19	Ulcer	UL	II	+	+	+	+	+
BUCP19	F	10	Ulcer	LL	I	+	+	-	+	+
BUCP20	F	53	Ulcer	LL	II	-	-	-	-	-
BUCP8	M	46	Plaque	UL	III	+	+	-	+	+
BUCP13	M	10	Plaque	UL	II	+	-	+	+	+
BUCP15	M	10	Plaque	LL	III	+	-	+	+	+
BUCP17	F	33	Plaque	LL	II	-	-	-	-	-
BUCP21	M	12	Plaque	UL	II	+	-	-	+	-
BUCP18	M	45	Oedema	UL	III	-	-	+	+	-
BUCP7	F	48	Nodule	UL	I	-	-	-	-	-
BUCP11	M	5	Nodule	LL	I	-	-	-	-	-
BUCP16	F	7	Nodule	LL	I	-	-	-	-	-

*Details of culture outcomes (defined as a positive M. ulcerans recovered for at least one of the different methods at any time point) on 21 BU confirmed participants evaluated by Tika-MGIT (Mycobacterium Growth Indicator Tube), Petroff-MGIT and Petroff-LJ (Löwenstein Jenssen) and compared with 16 S rRNA assay for viable M. ulcerans. (+) indicates a positive outcome, (-) indicates a negative outcome); (C) indicates all time point samples contaminated (Mu) indicates Mycobacterium ulcerans; LL- lower limb; UL- upper limb; GN- genitalia; AB- Abdominal region; BP- back

ISSN: 1471-2180