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Table 3 Effect of 5 mM addition of metal ions and EDTA on the pure asparaginase activity produced by F. falciforme AUMC 16563. Residual activity (%) results are expressed as the proportion of the activity in the tested inhibitory conditions, from the asparaginase activity in the control without inhibitors (means with different values between treatments in the same column are significantly different at p < 0.05)

From: L-asparaginase from the novel Fusarium falciforme AUMC 16563: extraction, purification, characterization, and cytotoxic effects on PC-3, HePG-2, HCT-116, and MCF-7 cell lines

Metal ions

Specific activity (U/mg)

Residual activity (%)

Control

5220.23d

100±0.0d

Na+

7548.45b

144.6±2.0b

K+

8091.35a

155±1.5a

Ca++

7308.32b

140±1.0b

Mg++

3654.16e

70±0.5e

Fe++

6201.63c

118.8±1.5c

Cu++

866.56i

16.6±0.5i

Zn++

2537.03g

48.6±0.5g

Mn++,

3236.54f

62.0±1.5ef

Ba++

2009.78h

38.5±1.0h

Co++

2871.13g

55±1.5fg

Cd++

756.93i

14.5±0.5i

EDTA++

5220.23d

100±1.0d