Table 2 Detection of BoAHV1 IgG levels using a commercial ELISA kit and our established method with selected cattle sera. Four peptides, including PV49, PV109, PV116, and another PV109 (in a 1:1:1:1 ratio), conjugated to the vector KLH, were coated onto 96-well plates at a dose of 20 µg per well to create the kit. BoAHV1 IgG levels in 44 cattle serum samples provided by a local farmer were detected using both the established kit and the commercial ELISA kit (BioStone Animal Health, Southlake, TX, USA, cat# 10074-05). The results, as determined by OD450 readings from both kits, are listed

From: A B-cell epitope on the bovine herpesvirus 1 (BoAHV1) viral protein gC, referred to as PV116, is implicated in the generation of antibodies and the development of an ELISA kit for the detection of the virus antibody