Table 1 Enrichment of selected phages by affinity panning. 1 × 1011 pfu (input) of phage library was employed for binding to a coatedBoAHV1 specific antibody in each found of panning. The eluted phages for each round of panning were tittered as output. Recovery rate was calculated using the formula (input/output) %
Round of panning | BoAHV1 Antiserum (µg/mL) | Tween-20 (%) | Phage input (pfu) | Phage output (pfu) | Recovery rate (%) |
|---|---|---|---|---|---|
1 | 80 | 0.1 | 1 × 1011 | 5.1 × 105 | 5.1 × 10− 6 |
2 | 60 | 0.5 | 1 × 1011 | 4.2 × 106 | 4.2 × 10− 5 |
3 | 40 | 0.5 | 1 × 1011 | 2.3 × 107 | 2.3 × 10− 4 |