Fig. 7

The recognization of viral protein gC by mouse serum prepared by immunization of indicated peptides PV116. MDBK (A) and A549 (B) cells were infected with BoAHV1 at an MOI of 1 for the specified time durations. Subsequently, cell lysates (approximately 30 µg of total proteins) were prepared and subjected to detection of viral proteins by Western blot using the mouse serum produced by immunization with peptide PV116. (C) MDBK cells in 6-well plates were transfected with either scrambled siRNA (200 pmol) or 53BP1-specific siRNAs (200 pmol). At 48 h after transfection, the cells were infected with BoAHV1(MOI = 1). After infection for 24 h, cell lysates were prepared and subjected to Western blot to detect viral proteins using the serum against PV116-KLH. (D and E) Viral particles were purified by ultracentrifugation at 20,000 rpm using a Beckman SW28 rotor for 1 h at 4 °C. In parallel, cell debris was also collected for use as a negative control. Both viral particles and cell debris were subjected to detection of viral proteins by Western blot using mouse sera produced by immunization with the peptide PV116-KLH (D) and KLH (E), respectively. The images presented are representative of three independent experiments