Fig. 5

The identification of synthesized peptides conjugated to KLH by dot blot using BoAHV1 IgG positive cattle serum. (A) The peptides including PP46, PP49, PP59, PP64, PP109, PP116, PV46, PV50, PV59, PV62, PV64, PV49, PV108, PV109, PV113, PV116, PV130, and PV133, were commercially synthesized, conjugated to the carrier KLH, and dot-loaded onto NC membranes. Purified virions and the vector KLH were used as positive and negative controls, respectively. Approximately 3.5 µg of total proteins were loaded onto each blot. After blocking with 5% non-fat milk in PBST, the membranes were incubated with sera from cattle 72, 189,119, and 200,178 (1:3000), followed by incubation with goat anti-bovine IgG-HRP (1:8000). The immune reaction was developed using Clarity Western ECL substrate. The images shown are representative of three independent experiments