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Fig. 2 | BMC Microbiology

Fig. 2

From: A B-cell epitope on the bovine herpesvirus 1 (BoAHV1) viral protein gC, referred to as PV116, is implicated in the generation of antibodies and the development of an ELISA kit for the detection of the virus antibody

Fig. 2

The identification of selected peptides displayed on phages with commercial available goat anti-BoAHV1 serum. Nitrocellulose (NC) membranes were coated with purified phages denoted by Phage-P46, Phage-P49, Phage-P50, Phage-P59, Phage-P62, Phage-P64, Phage-P65, Phage-P71, Phage-P76, Phage-P104, Phage-P108, Phage-P109, Phage-P113, Phage-P116, Phage-P130, and Phage-P133, respectively. Purified virions and a phage expressing an irrelevant peptide recognized by BVDV antibody were used as positive and negative controls, respectively. After blocking with 5% non-fat milk in PBS, the membranes were incubated with goat anti-BoAHV1 serum (1:5000), followed by incubation with donkey anti-goat IgG-HRP (1:8000). The immune reaction was developed using Clarity Western ECL substrate. The images shown are representative of two independent experiments

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BMC Microbiology

ISSN: 1471-2180

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