Table 3 Analysis of Variance (ANOVA) table for the optimization of nutrient media against S. aureus response surface quadratic model

Model

14

0.812129

0.058009

51.62

0.000

Linear

4

0.181135

0.045284

40.30

0.000

Starch (%)

1

0.067278

0.067278

59.87

0.000

Yeast Extract (%)

1

0.018572

0.018572

16.53

0.001

NaCl (%)

1

0.029974

0.029974

26.67

0.000

NH₄Cl (%)

1

0.031912

0.031912

28.40

0.000

Square

4

0.237708

0.059427

52.88

0.000

Starch (%) *Starch (%)

1

0.086389

0.086389

76.87

0.000

Yeast Extract (%) *Yeast Extract (%)

1

0.008479

0.008479

7.54

0.014

NaCl (%) *NaCl (%)

1

0.089036

0.089036

79.23

0.000

NH₄Cl (%) *NH₄Cl (%)

1

0.033396

0.033396

29.72

0.000

2-Way Interaction

6

0.346075

0.057679

51.33

0.000

Starch (%) *Yeast Extract (%)

1

0.112225

0.112225

99.86

0.000

Starch (%) *NaCl (%)

1

0.038025

0.038025

33.84

0.000

Starch (%) *NH₄Cl (%)

1

0.164025

0.164025

145.96

0.000

Yeast Extract (%) *NaCl (%)

1

0.002500

0.002500

2.22

0.155

Yeast Extract (%) *NH₄Cl (%)

1

0.028900

0.028900

25.72

0.000

NaCl (%) *NH₄Cl (%)

1

0.000400

0.000400

0.36

0.559

Error

16

0.017981

0.001124

Lack-of-Fit

9

0.010431

0.001159

1.07

0.473

Pure Error

7

0.007550

0.001079

Total

30

0.830110

S

R-sq

R-sq(adj)

R-sq(pred)

0.0335230

97.83%

95.94%

90.74%