Detection of Francisellaceae and the differentiation of main European F. tularensis ssp. holarctica strains (Clades) by new designed qPCR assays

Table 2 Specificity of the qPCR assays. Qualitative results of qPCR are indicated as positive ( +) or negative

Francisellaceae species	Strain	F-16S	B.6	B.12	B.71	Fth clade
Allofrancisella sp.	W12-1067	+	-	-	-	na
A. frigidaquae		+	-	-	-	na
A. guangzhouensis		+	-	-	-	na
A. inopinata		+	-	-	-	na
Parafrancisella adeliensis		+	-	-	-	na
Pseudofrancisella sp.	U-3452	+	-	-	-	na
Pseudofrancisella sp.	U-3454	+	-	-	-	na
F. endociliophora	FSC 1006	+	-	-	-	na
F. halioticida		+	-	-	-	na
F. hispaniensis	FSC 454	+	-	-	-	na
F. hispaniensis	3523	+	-	-	-	na
F. noatunensis		+	-	-	-	na
F. noatunensis		+	-	-	-	na
F. novicida	Fx1	+	-	-	+	na
F. novicida	U112	+	-	+	+	na
F. opportunistica		+	-	-	-	na
F. orientalis		+	-	-	-	na
F. persica		+	-	-	-	na
F. philomiragia	ATCC 25015	+	-	-	-	na
F. philomiragia	25,01ATCC 25016	+	-	-	-	na
F. philomiragia	ATCC 25017	+	-	-	-	na
F. philomiragia	ATCC 25018	+	-	-	-	na
F. philomiragia	18844	+	-	-	-	na
Ftm	FSC 147	+	+	+	+	na
Ftt	ATCC 6223	+	-	-	+	na
Fth	LVS	+	-	+	+	B.12/B.72
Fth	Fth-41	+	+	+	+	B.4
Fth	A-271	+	-	+	+	B.12/B.72
Fth	A-317	+	-	+	-	B.12/B.71
Fth	A-635	+	+	-	+	B.6
Fth	A-660	+	+	-	+	B.6
Fth	A-663	+	-	+	+	B.12/B.72
Fth	A-702	+	-	+	-	B.12/B.71
Fth	A-981	+	+	-	+	B.6
Fth	A-1007	+	+	-	+	B.6
Fth	A-1158	+	+	-	+	B.6
Fth	A-1308	+	-	+	+	B.12/B.72
Fth	A-1341	+	-	+	-	B.12/B.71
Fth	A-1559	+	-	+	+	B.12/B.72
Fth	A-2219	+	+	-	+	B.6
Fth	A-2255	+	-	+	-	B.12/B.71
Fth	A-2299	+	+	-	+	B.6
Fth	Ft-42	+	+	+	+	B.4
Fth	A-362–15	+	+	+	+	B.4
Bacillus cereus^a		-	-	-	-	na

^aAll other bacteria listed in Table S2 were negatively tested in all qPCR assays; na: not applicable; + : positive qPCR result with Ct-value below 40; -: negative PCR result without Ct-value

ISSN: 1471-2180