Effects of different wet distillers’ grains ratios on fermentation quality, nitrogen fractions and bacterial communities of total mixed ration silage

Table 4 Nutrient composition of total mixed ration silages across varying storage durations

Items¹⁾	Treatments²⁾ (T)		Days of ensiling (D)					SEM³⁾	P-value⁴⁾
Items¹⁾	Treatments²⁾ (T)		0	7	15	30	60	SEM³⁾	T	D	T × D
DM		T0	438^Aa	386^b	344^d	367^c	373^Ac	0.900	< 0.001	< 0.001	< 0.001
(g kg^− 1, FM)		T15	413^Ca	387^b	340^d	376^c	364^Bc
		T30	417^Ba	374^b	330^d	375^b	362^Bc
WSC		T0	71.1^Aa	20.2^b	21.5^b	23.0^b	24.2^b	0.435	< 0.001	< 0.001	< 0.001
(g kg^− 1, FM)		T15	55.1^Ba	22.3^b	22.2^b	22.7^b	25.4^b
		T30	20.9^Cb	20.4^b	19.4^b	23.5^ab	27.0^a
CP		T0	172	163	167	155	159	1.06	< 0.001	< 0.01	0.888
(g kg^− 1, FM)		T15	165	159	158	156	160
		T30	163	154	153	151	153
aNDF		T0	351	395	365	403	395	3.53	< 0.001	< 0.001	0.608
(g kg^− 1, FM)		T15	352	379	392	403	402
		T30	383	407	404	451	402
ADF		T0	188^Cc	225^ABa	191^Bbc	215^Bab	227^a	2.30	< 0.001	0.142	< 0.001
(g kg^− 1, FM)		T15	229^Ba	206^Ba	228^Aa	208^Ba	231^a
		T30	290^Aa	244^Abc	242^Ac	268^Aab	251^bc

Differences between mean values labeled with different superscripts within the same row (a-d) or the same column (A-C) are statistically significant (p < 0.05)
¹⁾ DM, dry matter; WSC, water soluble carbohydrate; CP, crude protein; aNDF, neutral detergent fiber; ADF, acid detergent fiber
²⁾ T0, total mixed ration (TMR) with 0% wet distillers’ grains (DM); T15, TMR with 15% wet distillers’ grains (DM); T30, TMR with 30% wet distillers’ grains (DM)
³⁾ SEM, standard error of the mean
⁴⁾ D, effect of ensiling day; T, effect of treatment; T × D, interaction between treatment and ensiling days

ISSN: 1471-2180