Table 1 Strains and plasmids used in this study
From: A strategy for controlling Hypervirulent Klebsiella pneumoniae: inhibition of ClpV expression
Strains or plasmids | Description | Source |
|---|---|---|
Strains | ||
hvKP13 | Wild-type K. pneumoniae isolated from sputum | This study |
hvKP13 ΔclpV | hvKP13 mutant with clpV gene deletion | This study |
hvKP13 C-ΔclpV | hvKP13 ΔclpV mutant carrying the pGEM-T-Easy-clpV plasmid | This study |
KP5-GFP | K. pneumoniae carrying the pTD103luxI-sfGFP plasmid for intraspecific competitive experiments | Laboratory construction |
Escherichia coli | Used for interspecific competitive experiments | Laboratory stock |
Salmonella | Used for interspecific competitive experiments | Laboratory stock |
Pseudomonas aeruginosa | Used for interspecific competitive experiments | Laboratory stock |
Staphylococcus aureus | Used for interspecific competitive experiments | Laboratory stock |
Plasmids | ||
pSGKP-apr | Plasmid expressing sgRNA in hvKP13 (Aprr) | Laboratory construction |
pCas-kp-hph | Thermosensitive plasmid expresses Cas9 and lambda Red proteins in hvKP13 (Hphr) | Laboratory construction |
pGEM-T-Easy- Apr | pGEM-T-Easy-derived plasmid(Aprr) | Laboratory construction |
pGEM-T-Easy-clpV | pGEM-T-Easy-derived plasmid, with the hvKP13 clpV gene for clpV expression (Aprr) | Laboratory construction |