Fig. 1
From: A strategy for controlling Hypervirulent Klebsiella pneumoniae: inhibition of ClpV expression
Effect of clpV gene deletion on the growth of hypervirulent Klebsiella pneumoniae. (A) Gel electrophoresis of identification of clpV deletion strain. M: 2000 bp DNA marker. 1 lane: negative control with the primer of hcp-F/hcp-R. 2 lane: the PCR of WT strain with the primer of hcp-F/hcp-R. 3 lane: the PCR of ΔclpV strain with the primer of hcp-F/hcp-R. 4 lane: negative control with the primer of vgrG-F/vgrG-R. 5 lane: the PCR of WT strain with the primer of vgrG-F/vgrG-R. 6 lane: the PCR of ΔclpV strain with the primer of vgrG-F/vgrG-R. (B) The growth curves of WT, ΔclpV, and C-ΔclpV strains. WT: wild-type strain. ΔclpV: clpV gene deletion strain. C-ΔclpV: clpV gene complementary strain