Fig. 2
Experimental design for assessing the V. parahaemolyticus distribution over infection of E. diaphana. Individual anemones (strain CC7) were kept in 12-well plates with 2 ml of FSW supplemented with 50 µg/mL of spectinomycin in each well. Controls are represented by Ø (anemone kept in FSW without added bacteria) and FSW-injected anemones (injection of 100 µL FSW). For infected anemones, 100 µL of the bacterial solution containing 108 CFU of Vp-GFP was seeded in the FSW for bathing or directly injected into the E. diaphana pedal disk. Plates were incubated at 27 °C. For each time point (6, 24, and 30 h), 12 anemones were sampled. Bacterial visualization and quantification were analyzed at 6, 24, and 30 h post-inoculation