Table 1 Gene-specific primers used to detect efflux pump genes in A. Baumannii isolates
Target gene | Primer Sequence (5` to 3`) | Amplicon size (bp) | Melting Temp. | CG content | Annealing temp. | Ref. | |||
|---|---|---|---|---|---|---|---|---|---|
AdeA | F R | TGCCCAATACGCCCAGAAAT GTCAGGCTCTAGCCGATGTC | 106 | 60.03 59.97 | 50% 60% | 58 °C | [24] | ||
AdeB | F R | AACGTCGACCTGAGCCATTT GTGCGACTTATCCTGGTGCT | 185 | 59.97 60.11 | 50% 55% | 58 °C | [25] | ||
TetA | F R | GCTACATCCTGCTTGCCTTC CATAGATCGCCGTGAAGAGG | 210 | 58.98 57.94 | 55% 55% | 56 °C | [26] | ||
TetB | F R | GTAAAGCGATCCCACCACCA ACCACCTCAGCTTCTCAACG | 586 | 60.04 59.97 | 55% 55% | 58 °C | [27] | ||