Fig. 1

Effect of n-3/n-6 PUFA ratio on the rumen fluid bacteria community. The chord diagram shows the contribution of different rumen fluid bacteria communities at the phylum level (A). Linear discriminant analysis (LDA) demonstrated the rumen fluid bacteria community between CON and HN3 groups from phylum to genus level in the prepartum (B) and the postpartum (C) period with P value < 0.05 and LDA score > 2. The interactions network among bacteria at the genus level for the CON (D) and the HN3 (E) group. (F) Random forest analysis of rumen fluid bacteria at the genus level, the greater the Mean Decrease Accuracy, the greater the contribution of the genus