Table 3 Summary of purification phases of the ChOx produced by E. fergusonii
Purification phases | Total activity (U/mL) | Protein content (mg/mL) | Specific activity (U/mg) | Purification folds | Yield (%) | |
|---|---|---|---|---|---|---|
Cell-free filtrate (crude) | 4827 | 4235 | 1.14 | 1.00 | 100 | |
Saturation | 3617 | 592 | 6.11 | 5.35 | 74.9 | |
Chromatography | 1287 | 93.0 | 15.8 | 13.86 | 26.6 | |