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Fig. 2 | BMC Microbiology

Fig. 2

From: The infectivity of virus particles from Wolbachia-infected Drosophila

Fig. 2

Wolbachia increases survival in flies, which is linked to a reduction in the accumulation of viral RNA during infection with Drosophila C virus or Flock House Virus. A. C. Survival and B. D. accumulation of viral RNA as measured by RT-qPCR in Drosophila melanogaster flies with (red) or without (black)Wolbachia following infection 50.6 nL of Drosophila C virus (DCV) A. and B. or Flock House Virus (FHV) C. and D. at 108 IU/mL or 109 IU/mL respectively in phosphate-buffered saline (PBS). A. and C. Kaplan-Meier survival curves and 95% confidence intervals (shaded (n = 3). Flies with and withoutWolbachiawere also mock-infected with PBS as a negative control (dashed lines). B. and D. Log10 transformed mean normalised expression of DCV or FHV genome relative to host gene RpL32 (n = 6). Lines representing LOESS regression and associated confidence interval (shaded). Time points where DCV RNA was lower in flies with Wolbachia are signified by *

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