The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response

Table 2 Oligonucleotide primers used in this study

Primer name	Oligonucleotide (5′-3′)^a	Product size/bp	Tm/℃
rt-16 S-f	TTTGAGTTCCCGGCC	259	60
rt-16 S-r	CGGCCGCAAGGTTAA	259	60
rt-oxyR-f	GGGAATGCTGCTGGTGGATC	210	60
rt-oxyR-R	GGGTCTGTGCTTCATGCAGA	210	60
rt-soxR-f	GGCGACCATTGGTGAAGCGT	180	60
rt-soxR-r	CAATCACTGCGCGAAAGGCA	180	60
rt-ycgV-f	AGCATCTTTTCCGGCGGTTC	187	60
rt-ycgV-r	AAATTCCCCTGGCTCCTGCC	187	60
rt-ahpC-f	AGCAGCTCTGAAACCATCGC	183	60
rt-ahpC-r	GTCACGGCCAATGCCTTCAG	183	60
rt-ahpF-f	AAACGTGCGGCAGAAGAGCT	199	60
rt-ahpF-r	GCCCTTCAGTCTTCGGTACA	199	60
rt-katG-f	GCGCAGATGCCATTACCTCT	171	60
rt-katG-r	ACGGATCCGGGATAATTTCC	171	60
rt-fepG-f	TGATTTACGTCTCTCGCC	180	60
rt-fepG-r	GTAAACGCCATTCGGTGA	180	60
rt-sodA-f	ACCACACCAAACACCATCAG	185	60
rt-sodA-r	ACCTTTCCAGAACAGGCTGT	185	60
ahpC-biotin-f	TCGAGTAAAAGGCATAACCT	341	50
ahpC-r	TATACTTCCTCCGTGTTTTC	341	50
katG-biotin-f	ATAGTGTGGCTTTTGTGAAA	328	50
katG-r	CAATGTGCTCCCCTCTACAG	328	50
kpsM-biotin-f	CCATTTGATGATGTGATCCT	287	50
kpsM-r	TTTTCTGAGAAATTAACTCT	287	50
oxyR-biotin-f	AACGGGCAGTGACTTCAAGG	140	50
oxyR-r	TATCCATCCTCCATCGCCAC	140	50
sodA-biotin-f	CTTCTTATCCTCATCATTTT	284	50
sodA-r	ATTCATCTCCAGTATTGTCG	284	50
soxR-biotin-f	ATCAATGTTAAGCGGCTGGT	160	50
soxR-r	AAATCGCTTTACCTCAAGTT	160	50
ycgV-biotin-f	ATTCTCTGAGAAGCTCATCA	126	50
ycgV-r	ACCACTCCTATATAGTACCC	126	50
fepG-biotin-f	CAATTGAGATGAAACGAG	201	50
fepG-r	ACGAACTTCCATGATAAT	201	50
lacZ-biotin-f	CTGGCCGTCGTTTTACAACG	199	50
lacZ-r	AGCTTTCCGGCACCGCTTCT	199	50

^aThe sequences with the underline refer to the restriction endonuclease recognition sites

ISSN: 1471-2180