Fig. 8

(A) B. cereus MSF4 PHB synthesis genes and comparison with other class IV PHB producers in the BV-BCR database. Strain MSF4 presented six PHB synthase genes related to butanoate metabolism: PHB synthase PhaC, repressor PhaR, Acetoacetyl-CoA reductase PhaB, Phasin protein PhaP, (R)-specific enoyl-CoA hydratase PhaJ, and repressor PhaQ, which are arranged in the same way as those in the cereus group. P. megaterium is part of a different subgroup of Class IV PHAs, but unlike Classes I, II and III, the figure shows all the genes of the PHB-producing cluster. (B) Maximum likelihood phylogenetic tree comparing the polyhydroxybutyrate synthase gene PhaC of each of the four classes. All Bacillus were grouped in class IV, Haloferax in group III, Pseudomonas in group II and Cupriavidus in group I