Fig. 2

Evaluation of rhAmp genotyping assays. The 892 (Panel a) and 1352 (Panel b) genotyping assays were initially tested with 1 and 5 ng of DNA from both Sterne and a western African isolate in multiple replicates. The red dots along the x axis illustrate the Sterne-specific genotype with 1 and 5 ng samples clustering closely. Replicates of the Nigerian isolate clustered at the top left corner of the y axis in a concentration-independent manner (blue cluster). Heterozygous controls, consisting of equivalent amounts of Sterne and Nigerian DNA, were tested and are depicted in green at the top right corner of each plot. Black squares near the plot origin represent negative controls