Fig. 6

Transmission capacity of A. brassicicola wild-type (WT) and MCC/eisosome mutants to Arabidopsis thaliana seeds (Ler ecotype). The seed transmission capacity according to the silique stage and global seed transmission capacity (strain model) were measured as described by [37]. The five oldest siliques of at least five plants were inoculated with each fungal genotype and the experiment was repeated . Contaminated siliques were harvested 10 dpi. After dissection, seeds were incubated separately on PDA medium for 2 days. A seed was considered contaminated when incubation resulted in typical A. brassicicola colony development. For each inoculated fungal genotype, the seed infection probability was evaluated from at least 1000 seeds. Values represent infection probabilities with 95% confidence interval