Table 1 Ingredients and preparation of the standard Luria Bertani (LB) agar media
Ingredients | Amounts (g) | Preparation procedures | |
|---|---|---|---|
1 | Yeast extract | 2.5 | Ingredients 1–4 were put in an Erlenmeyer containing 250 mL of distilled water and the solution was mixed with a magnetic stirrer. Then distilled water was added to total volume of 500 mL and transferred to 1 L flask. The liquid was autoclaved for 20 min at 115 °C and let to cool at ~ 55 °C before pipetting 25 mL onto each petri dish plate. |
2 | Tryptone | 5 | |
3 | NaCl | 5 | |
4 | Agar powder | 7.5 | |
5 | Water | 500 | |