Small-scale analysis of exopolysaccharides from Streptococcus thermophilus grown in a semi-defined medium

BMC Microbiology

Table 2 Comparison of strains in MST

Strain	μ_max (h^-1)	OD₆₂₀	GalN	Gal	Glc	Composition of repeating
			(μM)	(μM)	(μM)	unit^*
SY89	0.93 ± 0.06	2.7	1.2	7.7	5.9	2 Gal, 2 Glc
IMD04	0.44 ± 0.04	1.8	3.9	26.7	26.8	Unknown
EU10	0.63 ± 0.03	2.6	0.0	49.5	37.2	Unknown
SY102	0.62 ± 0.03	1.3	1.2	27.8	24.0	2 Gal, 2 Glc
LY03 (IMD01)	0.92 ± 0.08	2.0	3.8	8.6	4.2	1 GalN, 2 Gal, 1 Glc
DSM8713	0.96 ± 0.01	2.5	0.2	0.2	0.8	Unknown

Monomer concentrations after hydrolysis in 4 M HCl. EPSs were analysed at the optical density (OD) indicated. Abbreviations: μ_max, maximum specific growth rate; Glc, glucose; Gal, galactose; GalN, galactosamine. No rhamnose or glucosamine was found in the EPSs from any of the strains. ^*Composition of soluble EPS as determined by NMR [12]. The galactosamine is actually N-acetylgalactosamine, but the acetyl group is removed during hydrolysis.

ISSN: 1471-2180