Table 3 Bacterial strains and plasmids used or created in this study.

Strains

Escherichia coli

XL1-Blue

K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi

Laboratory collection

relA 1 lac ^- F' [proAB ⁺ lacI ^q lacZ Δ M15 Tn10(tet^R)]

Bacillus subtilis

168

trpC 2

[36]

BSIP7004

trpC 2 speD::spc

[2]

BFS1850

trpC 2mtnK::lacZ

This work^a

BFS1851

trpC 2mtnU::lacZ

This work^a

BSHP1850

trpC 2speD::spc mtnT::lacZ

This work

BSHP7010

trpC 2 mtnS::spc

This work

BSHP7035

trpC 2pmtnK::Tn10

This work

Plasmids

pUC19

cloning vector, Amp^R

[37]

pIC333

mini-Tn10 delivery vector, Spc^R, Ery^R

[32]

pMutin4mcs

cloning vector, Erm^R

[31]

pDU1850

pMutin4mcs::mtnK

This work

pDU1851

pMutin4mcs::mtnU

This work

pHPP7010

pUC19mtnS::spc ^b

This work